Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes of therapeutic proteins.

Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes of therapeutic proteins.

Biotechnol J. 2012 Aug 22;

Authors: Li X, de Roo G, Burgers K, Ottens M, Eppink M

Abstract
The use of high throughput screening (HTS) has successfully been applied in the past years in downstream process development of therapeutic proteins. Different HTS applications were introduced to speed up the purification process development of these proteins. In the light of these findings, studies were conducted to develop a controlled way of pipetting the right amount of resin in self-packed filter plates. In total thirteen plates were tested for suitability in a HTS application, based upon the different studies the Seahorse plate was experimentally verified as the best filter plate. Suitable conditions to prepare a self-packed plate were established so that linear correlations between resin concentration (POROS 50HS) and UV adsorption (420 nm) were obtained and the accuracy of slurry dispensing in a micro-titer plate was controlled within 1% deviation under established conditions. Overall, self packed filter plates are equipped to be used in the development of purification processes in HTS mode.

PMID: 22911664 [PubMed - as supplied by publisher]

peptide labeling peptide dye biotinylated peptide