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they potentiated the ability of another 5 HT, agonist, lisuride, to induce tail flicks and, in their presence, the S HT, receptor partial agonists flesinoxan and buspirone also induced tail flicks. These observations indicate the common and (-)-MK 801 robust nature with the potentiation with the tail flick response evoked by receptor agonists. Interestingly, BMY 7378 also induced tail flicks while in the presence of TFMPP. This obtaining is in line with recent reports that BMY 7378 displays residual partial agonist action at 5 HT,a ra:eptors. In contrast to BMY 7378, neither spiperone, NAN190 nor alprenolol, which might be pure S HTj receptor antagonists, elicited tail flicks, even while in the presence of TFMPP or DOI.

it seems unlikely that the measurement of DA, rather than endogenous DA, could account for the discrepancy between this study and that of Blandina et al.. Apart from its action in increasing basal tritium release, 5 HT also triggered an approximate 2 fold boost while in the calcium evoked release of tritium. In contrast, d LSD had no effect on stimulated tritium release. As using the boost in basal tritium efflux by 5 HT, the action of 5 HT on calcium A 205804 evoked tritium release was prevented from the uptake inhibitors cocaine and nomifensine. It was also partly antagonized by a higher concentration of imipramine. It therefore appears that like using the effect on basal release, 5 HT have to be taken up inside the dopaminergic terminal to be able to exert its effects on calcium evoked release.

The homogenate was centrifuged for 10 m in at 48,000 X g at 4 C, and the pellet was washed 3 occasions by resuspension in 10 volumes of buffer and centrifugation as above. The last pellet was resuspended in 20 volumes of ice cold 50 mM HEPES buffer, yielding about 2. 5 mg protein/ml suspension. Binding assays had been performed in 16 X one hundred mm polypropylene test tubes. Aliquots of 0. 4 ml with the cortical membrane suspension had been incubated for 30 PARP min at 25 C, in a last volume of 2 ml 50 mM HEPES buffer, while in the presence of 0. 3 0. 5 nM granisetron and 5 7 increasing concentrations with the inhibitory test drug. Non distinct binding was determined from samples incubated while in the presence of one hundred nM tropisetron or R,S zacopride. Incubations had been terminated by filtration more than Whatman GF/B filters which had been presoaked for 2 h in 0. 3% polyethylenimine in water. Filters had been then washed with 2 X 7. 5 ml of 50 mM HEPES buffer at space temperature, and immersed in 10 ml scintillation liquid.