Twelve Forecasts Over (-)-MK 801 Maleate A 205804 Next Year

we reported that tanshinone I and its congeners isolated from the roots of Salvia miltiorrhiza Bunge have memory enhancing and ameliorating effects on scopolamine induced memory impairment in mice. Furthermore, tanshinone I has also been reported to inhibit unitrazepam binding and to avert diazepam induced (-)-MK 801 Maleate memory decits.

These ndings suggest (-)-MK 801 Maleate that the antagonism shown by tanshinone I against diazepaminduced memory decits might not be directly derived from GABAA receptor blockade. We hypothesized that the memoryameliorating effect of tanshinone I against diazepam is not due to antagonism at GABAA receptors, but rather to the sharing or convergence of an intracellular signalling pathway, such as the ERK?CREB signalling pathway. In a pilot study, we found that tanshinone I and other tanshinone congeners, namely, tanshinone I, tanshinone IIA, cryptotanshinone and 15,16 dihydrotanshinone I, increased ERK phosphorylation within 1 h in normal mice. Here, we investigated the mode of action of tanshinone I with respect to ERK?CREB phosphorylation, and sought to determine whether tanshinone I treatment affects memory.

Passive avoidance performance was carried out in two identical light and dark square boxes separated by a NSCLC guillotine door, as described in our previous report. The illuminated compartment contained a 50 W bulb, and its oor was composed of 2 mm stainless steel rods spaced with centres 1 cm apart. A mouse was initially placed in the illuminated compartment for the acquisition trial, and the door between the two compartments was opened 10 s later. When the mouse entered the dark compartment, the guillotine door was automatically closed and an electrical foot shock of 3 s duration was delivered through the stainless steel rods. The mice were given tanshinone I 40 min before the acquisition trial. Memory impairment was induced by diazepam, a selective antagonist of the benzodiazepine site of the GABAA receptor or MK 801, an NMDA receptor channel blocker, which was administered 10 min after tanshinone I or vehicle.

Thereafter, we adopted 1 nmol for further study. U0126 was manually injected into lateral ventricle under anaesthesia, as previously described, A 205804 30 min before the acquisition trial, and animals were then returned to their home cages.